LASER SCANNING CONFOCAL MICROSCOPY

Laser scanning confocal microscopy (LSCM) also known as Confocal laser scanning microscopy (CLSM) or simply confocal microscopy, is a technique for obtaining high resolution optical images with depth selectivity. The key feature of confocal microscopy is its ability to acquire in-focus images from selected depths, a process known as optical sectioning. Images are acquired point-by-point and reconstructed with a computer. This allows three-dimensional reconstructions of topologically complex objects.