NETHERLANDS
The Van Leeuwenhoek Center for Advanced Microscopy (LCAM) - Functional Imaging Flagship Node Amsterdam
The Dutch LCAM – Functional Imaging flagship Node in Amsterdam in the Netherlands focuses on advanced life cell- and functional imaging and on technically challenging high-end experiments. This Node develops equipment, software and molecular constructs (fluorophores, small-molecule dyes and FRET sensors) to enable novel types of experiments. LCAM presents the single largest concentration of microscopy experts in the Dutch biomedical field with world-class specialists in e.g. FRET, FCCS, FLIM and instrumentation.
Offered Technologies:
| Technologies | Euro-BioImaging |
| Deconvolution widefield microscopy (DWM) | ✓ |
| Laser scanning confocal microscopy (LSCM/CLSM) | ✓ |
| Spinning disk confocal microscopy (SDCM) | ✓ |
| Two-photon microscopy (2P) | ✓ |
| Total internal reflection fluorescence microscopy (TIRF) | ✓ |
| Single Molecula localisation microscopy (SMLM) | ✓ |
| Light-sheet mesoscopic imaging (SPIM or dSLSM) | ✓ |
| High throughput microscopy/high content screening (HTM/HCS) | ✓ |
| Fluorescence (cross)-correlation spectroscopy (FCS/FCCS) | ✓ |
| Fluorescence Resonance Energy Transfer (FRET) | ✓ |
| Fluorescence Recovery After Photobleaching (FRAP) | ✓ |
| Fluorescence Lifetime Imaging Microscopy (FLIM) | ✓ |

Contact details
Dr. Mark Hink
Manager LCAM-FNWI
m.a.hink@uva.nl
http://www.lcam.nl/
Prof Dr. Kees Jalink
Manager LCAM-NKI
k.jalink@nki.nl